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code a1 300 code b1 r s n3 3  (New England Biolabs)


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    New England Biolabs code a1 300 code b1 r s n3 3
    Code A1 300 Code B1 R S N3 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/code+a1+300+code+b1+r+s+n3+3/a1-3/pmc07675038__ADVS___7___2001970___s001-223-79-104
    Average 93 stars, based on 18 article reviews
    code a1 300 code b1 r s n3 3 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. N3-Pool Step 1 NH2-Pool Step 1 DNAadaptor Ad1 Code B481-611 1x NEB buffer T4-DNA Ligase 2h, r.t. DNAadaptor Ad1 Code B1-480 1x NEB buffer T4-DNA Ligase 2h, r.t. … … N3 CONHRa1-300 NH O N3 CONHRa1-300 NH O N3 CONHRa1-300 NH O NH2 CONHRa1-300 NH O 6111-300 4811-300 4801-300 B NH2 CONHRa1-300 NH O NH2 CONHRa1-300 NH O 480 reactions BA BA BA A 131 reactions 1-300 11-300 24 a) b) c) NHO O N H 1-300 5’ 3’ code A1-300 code B1 (R,S) N3 3’ code B1 5’ NHO O N H 1-300 5’ code A1-300 (R,S) N3 3’ 3’ Ad1 5’ 1x NEB buffer T4-DNA Ligase 2h, r.t. + Ligase buffer 13 NH O BB1 A BB2 HN O* Code 1 Code 2 Adaptor Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 1.0e-3 2.0e-3 3.0e-3 4.0e-3 5.0e-3 6.0e-3 7.0e-3 8.0e-3 9.0e-3 1.0e-2 1.1e-2 1.2e-2 1.3e-2 1.4e-2 1.5e-2 1.6e-2 1.7e-2 (2) PDA Ch1 260nm@1.2nm Range: 2e-24.59Ligation product after HPLC Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 5.0e-3 1.0e-2 1.5e-2 2.0e-2 2.5e-2 3.0e-2 3.5e-2 4.0e-2 4.5e-2 5.0e-2 Ligation crude product (2) PDA Ch1 260nm@1.2nm Range: 6e-24.49 1.34 0.33 3.64 3.99 mass 18000 19000 20000 21000 22000 23000 24000 25000 26000 % 0 100 1: TOF MS ES3.85e523999.0000 21761.0000 18735.0000 20773.000019263.0000 19892.0000 22323.0000 23143.0000 23582.0000 24018.0000 25875.000024111.0000 25301.0000 25093.0000 26355.0000 26606.0000 Ligation product after HPLC deconv. (4.59) Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 1.0e-3 2.0e-3 3.0e-3 4.0e-3 5.0e-3 6.0e-3 7.0e-3 8.0e-3 9.0e-3 1.0e-2 1.1e-2 1.2e-2 1.3e-2 1.4e-2 1.5e-2 1.6e-2 1.7e-2 (2) PDA Ch1 260nm@1.2nm Range: 2e-24.59Ligation product after HPLC Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 5.0e-3 1.0e-2 1.5e-2 2.0e-2 2.5e-2 3.0e-2 3.5e-2 4.0e-2 4.5e-2 5.0e-2 Ligation crude product (2) PDA Ch1 260nm@1.2nm Range: 6e-24.49 1.34 0.33 3.64 3.99 mass 18000 19000 20000 21000 22000 23000 24000 25000 26000 % 0 100 1: TOF MS ES3.85e523999.0000 21761.0000 18735.0000 20773.000019263.0000 19892.0000 22323.0000 23143.0000 23582.0000 24018.0000 25875.000024111.0000 25301.0000 25093.0000 26355.0000 26606.0000 Ligation product after HPLC deconv. (4.59) Step 2 results : Ligation test Before HPLC after HPLC Supplementary figure 6: a) Model ligation on N3-pool step 1 and Code B1 used to test purification of the ligation product. b) RP-HPLC profile (graph in green) of the reaction crude registered at l=260 nm. ..

    Ligation:

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. N3-Pool Step 1 NH2-Pool Step 1 DNAadaptor Ad1 Code B481-611 1x NEB buffer T4-DNA Ligase 2h, r.t. DNAadaptor Ad1 Code B1-480 1x NEB buffer T4-DNA Ligase 2h, r.t. … … N3 CONHRa1-300 NH O N3 CONHRa1-300 NH O N3 CONHRa1-300 NH O NH2 CONHRa1-300 NH O 6111-300 4811-300 4801-300 B NH2 CONHRa1-300 NH O NH2 CONHRa1-300 NH O 480 reactions BA BA BA A 131 reactions 1-300 11-300 24 a) b) c) NHO O N H 1-300 5’ 3’ code A1-300 code B1 (R,S) N3 3’ code B1 5’ NHO O N H 1-300 5’ code A1-300 (R,S) N3 3’ 3’ Ad1 5’ 1x NEB buffer T4-DNA Ligase 2h, r.t. + Ligase buffer 13 NH O BB1 A BB2 HN O* Code 1 Code 2 Adaptor Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 1.0e-3 2.0e-3 3.0e-3 4.0e-3 5.0e-3 6.0e-3 7.0e-3 8.0e-3 9.0e-3 1.0e-2 1.1e-2 1.2e-2 1.3e-2 1.4e-2 1.5e-2 1.6e-2 1.7e-2 (2) PDA Ch1 260nm@1.2nm Range: 2e-24.59Ligation product after HPLC Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 5.0e-3 1.0e-2 1.5e-2 2.0e-2 2.5e-2 3.0e-2 3.5e-2 4.0e-2 4.5e-2 5.0e-2 Ligation crude product (2) PDA Ch1 260nm@1.2nm Range: 6e-24.49 1.34 0.33 3.64 3.99 mass 18000 19000 20000 21000 22000 23000 24000 25000 26000 % 0 100 1: TOF MS ES3.85e523999.0000 21761.0000 18735.0000 20773.000019263.0000 19892.0000 22323.0000 23143.0000 23582.0000 24018.0000 25875.000024111.0000 25301.0000 25093.0000 26355.0000 26606.0000 Ligation product after HPLC deconv. (4.59) Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 1.0e-3 2.0e-3 3.0e-3 4.0e-3 5.0e-3 6.0e-3 7.0e-3 8.0e-3 9.0e-3 1.0e-2 1.1e-2 1.2e-2 1.3e-2 1.4e-2 1.5e-2 1.6e-2 1.7e-2 (2) PDA Ch1 260nm@1.2nm Range: 2e-24.59Ligation product after HPLC Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 5.0e-3 1.0e-2 1.5e-2 2.0e-2 2.5e-2 3.0e-2 3.5e-2 4.0e-2 4.5e-2 5.0e-2 Ligation crude product (2) PDA Ch1 260nm@1.2nm Range: 6e-24.49 1.34 0.33 3.64 3.99 mass 18000 19000 20000 21000 22000 23000 24000 25000 26000 % 0 100 1: TOF MS ES3.85e523999.0000 21761.0000 18735.0000 20773.000019263.0000 19892.0000 22323.0000 23143.0000 23582.0000 24018.0000 25875.000024111.0000 25301.0000 25093.0000 26355.0000 26606.0000 Ligation product after HPLC deconv. (4.59) Step 2 results : Ligation test Before HPLC after HPLC Supplementary figure 6: a) Model ligation on N3-pool step 1 and Code B1 used to test purification of the ligation product. b) RP-HPLC profile (graph in green) of the reaction crude registered at l=260 nm. ..

    Purification:

    Article Title: A Single‐Stranded DNA‐Encoded Chemical Library Based on a Stereoisomeric Scaffold Enables Ligand Discovery by Modular Assembly of Building Blocks
    Article Snippet: .. N3-Pool Step 1 NH2-Pool Step 1 DNAadaptor Ad1 Code B481-611 1x NEB buffer T4-DNA Ligase 2h, r.t. DNAadaptor Ad1 Code B1-480 1x NEB buffer T4-DNA Ligase 2h, r.t. … … N3 CONHRa1-300 NH O N3 CONHRa1-300 NH O N3 CONHRa1-300 NH O NH2 CONHRa1-300 NH O 6111-300 4811-300 4801-300 B NH2 CONHRa1-300 NH O NH2 CONHRa1-300 NH O 480 reactions BA BA BA A 131 reactions 1-300 11-300 24 a) b) c) NHO O N H 1-300 5’ 3’ code A1-300 code B1 (R,S) N3 3’ code B1 5’ NHO O N H 1-300 5’ code A1-300 (R,S) N3 3’ 3’ Ad1 5’ 1x NEB buffer T4-DNA Ligase 2h, r.t. + Ligase buffer 13 NH O BB1 A BB2 HN O* Code 1 Code 2 Adaptor Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 1.0e-3 2.0e-3 3.0e-3 4.0e-3 5.0e-3 6.0e-3 7.0e-3 8.0e-3 9.0e-3 1.0e-2 1.1e-2 1.2e-2 1.3e-2 1.4e-2 1.5e-2 1.6e-2 1.7e-2 (2) PDA Ch1 260nm@1.2nm Range: 2e-24.59Ligation product after HPLC Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 5.0e-3 1.0e-2 1.5e-2 2.0e-2 2.5e-2 3.0e-2 3.5e-2 4.0e-2 4.5e-2 5.0e-2 Ligation crude product (2) PDA Ch1 260nm@1.2nm Range: 6e-24.49 1.34 0.33 3.64 3.99 mass 18000 19000 20000 21000 22000 23000 24000 25000 26000 % 0 100 1: TOF MS ES3.85e523999.0000 21761.0000 18735.0000 20773.000019263.0000 19892.0000 22323.0000 23143.0000 23582.0000 24018.0000 25875.000024111.0000 25301.0000 25093.0000 26355.0000 26606.0000 Ligation product after HPLC deconv. (4.59) Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 1.0e-3 2.0e-3 3.0e-3 4.0e-3 5.0e-3 6.0e-3 7.0e-3 8.0e-3 9.0e-3 1.0e-2 1.1e-2 1.2e-2 1.3e-2 1.4e-2 1.5e-2 1.6e-2 1.7e-2 (2) PDA Ch1 260nm@1.2nm Range: 2e-24.59Ligation product after HPLC Time -0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 6.00 6.50 7.00 7.50 A U 0.0 5.0e-3 1.0e-2 1.5e-2 2.0e-2 2.5e-2 3.0e-2 3.5e-2 4.0e-2 4.5e-2 5.0e-2 Ligation crude product (2) PDA Ch1 260nm@1.2nm Range: 6e-24.49 1.34 0.33 3.64 3.99 mass 18000 19000 20000 21000 22000 23000 24000 25000 26000 % 0 100 1: TOF MS ES3.85e523999.0000 21761.0000 18735.0000 20773.000019263.0000 19892.0000 22323.0000 23143.0000 23582.0000 24018.0000 25875.000024111.0000 25301.0000 25093.0000 26355.0000 26606.0000 Ligation product after HPLC deconv. (4.59) Step 2 results : Ligation test Before HPLC after HPLC Supplementary figure 6: a) Model ligation on N3-pool step 1 and Code B1 used to test purification of the ligation product. b) RP-HPLC profile (graph in green) of the reaction crude registered at l=260 nm. ..



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    code a1 300 code b1 r s n3 3  (New England Biolabs)
    93
    New England Biolabs code a1 300 code b1 r s n3 3
    Code A1 300 Code B1 R S N3 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/code+a1+300+code+b1+r+s+n3+3/a1-3/pmc07675038__ADVS___7___2001970___s001-223-79-104
    Average 93 stars, based on 1 article reviews
    code a1 300 code b1 r s n3 3 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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